GWIPS-viz Forums / Ribosome Profiling / Ribosome profiling protocol / phiX

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phiX
20-Aug-2013, 08:31 AM
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phiX
What would be the optimal concentration of phiX control for sequencing ribosome footprints? Illumina recommends at least 1%, but up to 40% when base composition is skewed and when there is low sequence diversity in the library. How critical is this?
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