04-Oct-2017, 04:04 PM
Hi everyone,
first of all thank you for the RiboGalaxy tools, I am trying to analyze my RP data but I am a beginner in bioinformatic analysis so I found it very useful!
I have a (probably silly) question for you. I have 2 conditions 3 replicates each. When I prepare the riboSeqR input using the appropriate tool which is the best way to proceed? Choosing one "Ribo seq alignment" and the 3 corresponding replicates SAM file together or it is better to run one SAM file (so 1 replicate) at time?
Thank you in advance for the reply
Rosario
first of all thank you for the RiboGalaxy tools, I am trying to analyze my RP data but I am a beginner in bioinformatic analysis so I found it very useful!
I have a (probably silly) question for you. I have 2 conditions 3 replicates each. When I prepare the riboSeqR input using the appropriate tool which is the best way to proceed? Choosing one "Ribo seq alignment" and the 3 corresponding replicates SAM file together or it is better to run one SAM file (so 1 replicate) at time?
Thank you in advance for the reply
Rosario