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RUST for dwell time
20-Jul-2017, 09:23 AM
Post: #1
RUST for dwell time
Hi,
I am doing my first steps in RP analysis and I find your galaxy platform to be a very useful solution which allows a relatively smooth entry into a complex field. Thank you.
I am trying to use RUST to assess “dwell time” per codon in a sample of interest.
If I understand correctly, I should be using the metafootprint analysis feature at the codon level.
The output I get are two plots as in figure 5 in the nature communication paper (https://www.nature.com/articles/ncomms12915).
The first plot shows RUST ratio (log2) and is useful to decide on read length and offset for A site assignment.
The actual data for this plot is available as another output file.

I believe the second plot is what I need, in that the values per codon should represent dwell time. My questions are:
1. The Y axis for the second plot states: "RUST ration relative to min". what does relative to min means?
2. Where can I find the actual values used for this plot. I was expecting to get a table like for I got for the first plot.
3. Alternatively, can I use the values from the data table of the first plot to calculate the values for the second plot
Many thanks,
Amir
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Messages In This Thread
RUST for dwell time - Amire - 20-Jul-2017 09:23 AM
RE: RUST for dwell time - Patrick O' Connor - 20-Jul-2017, 12:45 PM
RE: RUST for dwell time - Amire - 20-Jul-2017, 01:09 PM

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