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Ribo-SeqR input preparation
04-Oct-2017, 04:21 PM
Post: #2
RE: Ribo-SeqR input preparation
Hi Rosario,

It is a good question.

I think it would be best to initially run the riboSeqR tool on each SAM file separately so that you can get a sense if the triplet periodicity/metagene analysis is similar across the individual samples. This would also help determine if there are any issues.

If your intention is to then use the differential translation analysis component of riboSeqR (baySeq), then you would need to re-do the Prepare riboSeqR input, Triplet Periodicity and Metagene Analysis steps. In the Prepare riboSeqR input step, include the 3 corresponding replicate SAM files for each replicate group.

If you encounter any issues let us know.

All the best,
Audrey
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RE: Ribo-SeqR input preparation - audrey - 04-Oct-2017 04:21 PM

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