Why is ribosomal rRNA depletion optional for riboseq protocols
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30-Aug-2019, 01:15 AM
(This post was last modified: 30-Aug-2019 01:19 AM by Winwu1234.)
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Why is ribosomal rRNA depletion optional for riboseq protocols
Hi everyone,
First of all, just want to say that the Ribogalaxy platform has been very useful! I am in the process of optimising my Ribo-seq experimental protocol and I noted some papers/protocols do not incorporate a rRNA depletion step when extracting their RPFs. This may be a silly question, but I was wondering why is this the case and what kind of information can still be gathered if you do not deplete rRNA? Thanks |
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