Description fromGSE102113: Detection of acetylated cytide in RNA with immunoprecipitation (RIP), along with RNA-Seq, Ribo-Seq, and BRIC-Seq to analyze effects on RNA processing and stability.
Raw sequence data were obtainedfrom NCBI FTP directory(SRP114504).
Data from the following samples were processed:
Adapter sequence CTGTAGGCACCATCAAT and AGATCGGAAGAGCACAC was removed from reads using Cutadapt An alignment to ribosomal RNA was performed using Bowtie, and aligning reads were discarded. An alignment to the hg38 genome assembly was performed using Bowtie, and reads below 25 nt were discarded.
These tracks contain the uniquely mapping reads.,
| NAT10_RNASeq ||mRNA-seq unique mappers Homo sapiens(Arango, et al. 2018) |
| HeLa_RNASeq ||mRNA-seq unique mappers Homo sapiens(Arango, et al. 2018) |
Arango D, Sturgill D, Alhusaini N, Dillman AA, Sweet TJ, Hanson G, Hosogane M, Sinclair WR, Nanan KK, Mandler MD, Fox SD, Zengeya TT, Andresson T, Meier JL, Coller J, Oberdoerffer S (2018) Acetylation of Cytidine in mRNA Promotes Translation Efficiency. .Cell. 2018 Nov 12. pii: S0092-8674(18)31383-7. doi: 10.1016/j.cell.2018.10.030. [Epub ahead of print]