HEK293: Gao14 Track Settings
 
Quantitative profiling of initiating ribosomes in vivo (Xiangwei et al. 2014)   (All mRNA-seq Reads tracks)

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     SRR1630838  mRNA-seq unique mappers (SRR1630838) (Xiangwei et al. 2014)   Schema 
     SRR1630839  mRNA-seq unique mappers (SRR1630839) (Xiangwei et al. 2014)   Schema 
     SRR1630840  mRNA-seq unique mappers (SRR1630840) (Xiangwei et al. 2014)   Schema 

Methods

Data from the following samples were processed:
SRR1630838 mRNA-seq unique mappers Homo sapiens(Xiangwei, et al. 2014)
SRR1630839 mRNA-seq unique mappers Homo sapiens(Xiangwei, et al. 2014)
SRR1630840 mRNA-seq unique mappers Homo sapiens(Xiangwei, et al. 2014)

Adapter sequence n was removed from reads using Cutadapt An alignment to ribosomal RNA was performed using Bowtie, and aligning reads were discarded. An alignment to the hg38 genome assembly was performed using Bowtie, and reads below 25 nt were discarded. These tracks contain the uniquely mapping reads.,

References

Xiangwei Gao, Ji Wan, Botao Liu, Ming Ma, Ben Shen and Shu-Bing Qian (2014) Quantitative profiling of initiating ribosomes in vivo.doi: 10.1038/nmeth.3208