Ribosome profiling of lactimidomycin and cycloheximide treated human Jurkat T-lymphocytes
Raw sequence data were obtained from NCBI FTP directory(SRP065022). Data from the following samples were processed:
Adapter sequence CTGTAGGCACCATCAAT was removed from reads using Cutadapt An alignment to ribosomal RNA was performed using Bowtie, and aligning reads were discarded. An alignment to the hg38 genome assembly was performed using Bowtie, and reads below 25nt were discarded. These tracks contains the uniquely mapping reads.An offset of 12 nucleotides was then used to get the corresponding P-site of each read.
| Lactimidomycin_treated_Jurkat_cells ||Ribosome profiling data from Homo sapiens(Gawron, et al. 2016) |
Gawron D, Ndah E, Gevaert K, Van Damme P (2016) Positional proteomics reveals differences in N-terminal proteoform stability. . Mol Syst Biol. 2016 Feb 18;12(2):858. doi: 10.15252/msb.20156662.