Immortalized human lymphocytes were analyzed by ribosome profiling protocols with or without ribosome purification
Raw sequence data were obtained from NCBI FTP directory(SRP057384). Data from the following samples were processed:
Adapter sequence AGATCGGAAGAGCACAC was removed from reads using Cutadapt An alignment to ribosomal RNA was performed using Bowtie, and aligning reads were discarded. An alignment to the hg38 genome assembly was performed using Bowtie, and these tracks contains the uniquely mapping reads.An offset of 15 nucleotides was then used to get the corresponding A-site of each read.
| lympho_directExtract ||Ribosome profiling data from Homo sapiens(Reid, et al. 2015) |
| lympho_pelleted ||Ribosome profiling data from Homo sapiens(Reid, et al. 2015) |
Reid DW, Shenolikar S, Nicchitta CV (2015) Simple and inexpensive ribosome profiling analysis of mRNA translation. . Methods. 2015 Dec;91:69-74. doi: 10.1016/j.ymeth.2015.07.003. Epub 2015 Jul 8. Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't