RPF and RNAseq libraries were generated from mock or IFN-g-primed human macrophages. Cells were stimulated with Pam3Cys and harvested at 4 hours. Libraries were generated using protocol modified from Illumina Truseq technology.
Raw sequence data were obtained from NCBI FTP directory(SRP056098). Data from the following samples were processed:
Adapter sequence AAAAAAAAAAAAA was removed from reads using Cutadapt An alignment to ribosomal RNA was performed using Bowtie, and aligning reads were discarded. An alignment to the hg38 genome assembly was performed using Bowtie, and reads below 25nt were discarded. These tracks contains the uniquely mapping reads.
| IFNg_RNA ||mRNA-seq unique mappers from Homo sapiens(Su, et al. 2015) |
| Mock_RNA ||mRNA-seq unique mappers from Homo sapiens(Su, et al. 2015) |
Su X, Yu Y, Zhong Y, Giannopoulou EG, Hu X, Liu H, Cross JR, Ratsch G, Rice CM, Ivashkiv LB (2015) Interferon-γ regulates cellular metabolism and mRNA translation to potentiate macrophage activation. . Nat Immunol. 2015 Aug;16(8):838-49. doi: 10.1038/ni.3205. Epub 2015 Jun 29. Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't