RPE1: Tanenbaum 2015 Track Settings

Regulation of mRNA translation during mitosis (Tanenbaum et al. 2015) (All Elongating Ribosomes (Footprints) tracks)

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	G1cells	Ribosome Coverage of elongating ribosomes from Homo Sapiens (Tanenbaum et al. 2015)	Schema
	G2cells	Ribosome Coverage of elongating ribosomes from Homo Sapiens (Tanenbaum et al. 2015)	Schema
	Mcells	Ribosome Coverage of elongating ribosomes from Homo Sapiens (Tanenbaum et al. 2015)	Schema

Description

Ribosome profiling and mRNA-seq from 3 time points in the cell cycle.

Methods

Raw sequence data were obtained from NCBI FTP directory(SRP057253). Data from the following samples were processed:

Mcells	Ribosome coverage data from Homo Sapiens,Mcells (Tanenbaum, et al. 2015)
G1cells	Ribosome coverage data from Homo Sapiens, G1cells (Tanenbaum, et al. 2015)
G2cells	Ribosome coverage data from Homo Sapiens, G2cells (Tanenbaum, et al. 2015)

Adapter sequence CTGTAGGCACCATCAAT was removed from reads using Cutadapt. An alignment to ribosomal RNA was performed using Bowtie, and aligning reads were discarded. An alignment to the hg38 genome assembly was performed usingBowtie, and these tracks contains the uniquely mapping reads.

References

Tanenbaum ME, Stern-Ginossar N, Weissman JS, Vale RD (2015) Regulation of mRNA translation during mitosis . Science. doi: 10.7554/eLife.07957