Ribosome profiling and mRNA-seq from 3 time points in the cell cycle.
Raw sequence data were obtained from NCBI FTP directory(SRP057253). Data from the following samples were processed:
Adapter sequence CTGTAGGCACCATCAAT was removed from reads using Cutadapt. An alignment to ribosomal RNA was performed using Bowtie, and aligning reads were discarded. An alignment to the hg38 genome assembly was performed usingBowtie, and these tracks contains the uniquely mapping reads.An offset of 15 nucleotides was then used to get the corresponding A-site of each read.
| G2cells ||Ribosome profiling data from Homo Sapiens, G2cells (Tanenbaum, et al. 2015) |
| Mcells ||Ribosome profiling data from Homo Sapiens, Mcells (Tanenbaum, et al. 2015) |
| G1cells ||Ribosome profiling data from Homo Sapiens, G1cells (Tanenbaum, et al. 2015) |
Tanenbaum ME, Stern-Ginossar N, Weissman JS, Vale RD (2015) Regulation of mRNA translation during mitosis . Science. doi: 10.7554/eLife.07957