Description Examination of RNA fragments protected by ribosome MethodsRaw sequence data were obtained from NCBI FTP directory(SRP063852). Data from the following samples were processed:
| ribosome_profiling | Ribosome coverage data from Homo sapiens(Calviello, et al. 2016) |
Adapter sequences had already been removed from reads. An alignment to ribosomal RNA was performed using Bowtie, and aligning reads were discarded. An alignment to the hg38 genome assembly was performed using Bowtie, and reads below 25nt were discarded. These tracks contains the uniquely mapping reads.
References
Calviello L, Mukherjee N, Wyler E, Zauber H, Hirsekorn A, Selbach M, Landthaler M, Obermayer B, Ohler U (2016) Detecting actively translated open reading frames in ribosome profiling data. . Nat Methods. 2016 Feb;13(2):165-70. doi: 10.1038/nmeth.3688. Epub 2015 Dec 14. Research Support, N.I.H., Extramural
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