HEK293: Gao14 Track Settings

Quantitative profiling of initiating ribosomes in vivo (Xiangwei et al. 2014) (All Elongating Ribosomes (A-site) tracks)

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	SRR1630831	Ribosome profiles from elongating ribosomes (SRR1630831) (Xiangwei et al. 2014)	Schema
	SRR1630832	Ribosome profiles from elongating ribosomes (SRR1630832) (Xiangwei et al. 2014)	Schema
	SRR1630833	Ribosome profiles from elongating ribosomes (SRR1630833) (Xiangwei et al. 2014)	Schema

Methods

Data from the following samples were processed:

SRR1630831	Ribosome profiles from elongating ribosomes Homo sapiens(Xiangwei, et al. 2014)
SRR1630832	Ribosome profiles from elongating ribosomes Homo sapiens(Xiangwei, et al. 2014)
SRR1630833	Ribosome profiles from elongating ribosomes Homo sapiens(Xiangwei, et al. 2014)

Adapter sequence n was removed from reads using Cutadapt An alignment to ribosomal RNA was performed using Bowtie, and aligning reads were discarded. An alignment to the hg38 genome assembly was performed using Bowtie, and reads below 25 nt were discarded. An offset of 15 nucleotides was then used to get the corresponding A-site of each read.These tracks contain the uniquely mapping reads.,

References

Xiangwei Gao, Ji Wan, Botao Liu, Ming Ma, Ben Shen and Shu-Bing Qian (2014) Quantitative profiling of initiating ribosomes in vivo.doi: 10.1038/nmeth.3208