HEK293: Gao14 Track Settings

Quantitative profiling of initiating ribosomes in vivo (Xiangwei et al. 2014) (All Initiating Ribosomes (P-site) tracks)

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	SRR1630828	Ribosome profiles from initiating ribosomes (SRR1630828) (Xiangwei et al. 2014)	Schema
	SRR1630829	Ribosome profiles from initiating ribosomes (SRR1630829) (Xiangwei et al. 2014)	Schema
	SRR1630830	Ribosome profiles from initiating ribosomes (SRR1630830) (Xiangwei et al. 2014)	Schema

Methods

Data from the following samples were processed:

SRR1630828	Ribosome profiles from initiating ribosomes Homo sapiens(Xiangwei, et al. 2014)
SRR1630829	Ribosome profiles from initiating ribosomes Homo sapiens(Xiangwei, et al. 2014)
SRR1630830	Ribosome profiles from initiating ribosomes Homo sapiens(Xiangwei, et al. 2014)

Adapter sequence n was removed from reads using Cutadapt An alignment to ribosomal RNA was performed using Bowtie, and aligning reads were discarded. An alignment to the hg38 genome assembly was performed using Bowtie, and reads below 25 nt were discarded. An offset of 12 nucleotides was then used to get the corresponding P-site of each read.These tracks contain the uniquely mapping reads.,

References

Xiangwei Gao, Ji Wan, Botao Liu, Ming Ma, Ben Shen and Shu-Bing Qian (2014) Quantitative profiling of initiating ribosomes in vivo.doi: 10.1038/nmeth.3208