Jurkat: Gawron 2016 Track Settings

Positional proteomics reveals differences in N-terminal proteoform stability. (Gawron et al. 2016) (All Elongating Ribosomes (Footprints) tracks)

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	Cycloheximide treated Jurkat cells	Ribosome Coverage of ribosomes from Homo sapiens (Gawron et al. 2016)	Schema
	Lactimidomycin treated Jurkat cells	Ribosome Coverage of ribosomes from Homo sapiens (Gawron et al. 2016)	Schema

Description

Ribosome profiling of lactimidomycin and cycloheximide treated human Jurkat T-lymphocytes

Methods

Raw sequence data were obtained from NCBI FTP directory(SRP065022). Data from the following samples were processed:

Cycloheximide_treated_Jurkat_cells	Ribosome coverage data from Homo sapiens(Gawron, et al. 2016)
Lactimidomycin_treated_Jurkat_cells	Ribosome coverage data from Homo sapiens(Gawron, et al. 2016)

Adapter sequence CTGTAGGCACCATCAAT was removed from reads using Cutadapt An alignment to ribosomal RNA was performed using Bowtie, and aligning reads were discarded. An alignment to the hg38 genome assembly was performed using Bowtie, and reads below 25nt were discarded. These tracks contains the uniquely mapping reads.

References

Gawron D, Ndah E, Gevaert K, Van Damme P (2016) Positional proteomics reveals differences in N-terminal proteoform stability. . Mol Syst Biol. 2016 Feb 18;12(2):858. doi: 10.15252/msb.20156662.