Description Ribosome profiling of lactimidomycin and cycloheximide treated human Jurkat T-lymphocytes MethodsRaw sequence data were obtained from NCBI FTP directory(SRP065022). Data from the following samples were processed:
| Cycloheximide_treated_Jurkat_cells | Ribosome profiling data from Homo sapiens(Gawron, et al. 2016) |
Adapter sequence CTGTAGGCACCATCAAT was removed from reads using Cutadapt An alignment to ribosomal RNA was performed using Bowtie, and aligning reads were discarded. An alignment to the hg38 genome assembly was performed using Bowtie, and reads below 25nt were discarded. These tracks contains the uniquely mapping reads.An offset of 15 nucleotides was then used to get the corresponding A-site of each read.
References
Gawron D, Ndah E, Gevaert K, Van Damme P (2016) Positional proteomics reveals differences in N-terminal proteoform stability. . Mol Syst Biol. 2016 Feb 18;12(2):858. doi: 10.15252/msb.20156662.
|
|