Description Description fromGSE93133: Ribosome profiling libraries from haploid human cells from both wt and METTL21B knockouts.
Methods Raw sequence data were obtainedfrom NCBI FTP directory(SRP096025).
Data from the following samples were processed:
| METTL21B | Ribosome coverage data from elongating ribosomes Homo sapiens(Malecki, et al. 2017) |
| WT | Ribosome coverage data from elongating ribosomes Homo sapiens(Malecki, et al. 2017) |
Adapter sequences had already been removed from reads. An alignment to ribosomal RNA was performed using Bowtie, and aligning reads were discarded. An alignment to the hg38 genome assembly was performed using Bowtie, and reads below 25nt were discarded.
These tracks contain the uniquely mapping reads.,ReferencesMalecki J, Aileni VK, Ho AY, Schwarz J, Moen A, Sorensen V, Nilges BS, Jakobsson ME, Leidel SA, Falnes P.O (2017) The novel lysine specific methyltransferase METTL21B affects mRNA translation through inducible and dynamic methylation of Lys-165 in human eukaryotic elongation factor 1 alpha (eEF1A).Nucleic Acids Res. 2017 Jan 20. pii: gkx002. doi: 10.1093/nar/gkx002. [Epub ahead of print]
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