Description HeLa cells were synchronized at S or M phase, and subject to RNA-seq, ribosome profiling and TAIL-seq analysis. MethodsRaw sequence data were obtained from NCBI FTP directory(SRP072459). Data from the following samples were processed:
| S_phase_ribosome_profiling | Ribosome profiling data from Homo sapiens(Park, et al. 2016) |
| M_phase_ribosome_profiling | Ribosome profiling data from Homo sapiens(Park, et al. 2016) |
| Asynchronous_cell_ribosome_profiling | Ribosome profiling data from Homo sapiens(Park, et al. 2016) |
| M_phase_ribosome_profiling_synchronized_by_shake_off | Ribosome profiling data from Homo sapiens(Park, et al. 2016) |
Adapter sequence TGGAATTCTCGGGTGCC was removed from reads using Cutadapt An alignment to ribosomal RNA was performed using Bowtie, and aligning reads were discarded. An alignment to the hg38 genome assembly was performed using Bowtie, and reads below 25nt were discarded. These tracks contains the uniquely mapping reads.An offset of 15 nucleotides was then used to get the corresponding A-site of each read.
References
Park JE, Yi H, Kim Y, Chang H, Kim VN (2016) Regulation of Poly(A) Tail and Translation during the Somatic Cell Cycle. . Mol Cell. 2016 May 5;62(3):462-71. doi: 10.1016/j.molcel.2016.04.007.
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