RPE1: Tanenbaum 2015 Track Settings
 
Regulation of mRNA translation during mitosis (Tanenbaum et al. 2015)   (All mRNA-seq Reads tracks)

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     G1cells  mRNA Coverage from Homo Sapiens cell line: G1cells (Tanenbaum et al. 2015)   Schema 
     G2cells  mRNA Coverage from Homo Sapiens cell line: G2cells (Tanenbaum et al. 2015)   Schema 
     Mcells  mRNA Coverage from Homo Sapiens cell line: Mcells (Tanenbaum et al. 2015)   Schema 

Description

Ribosome profiling and mRNA-seq from 3 time points in the cell cycle.

Methods

Raw sequence data were obtained from NCBI FTP directory(SRP057253). Data from the following samples were processed:

G2cells mRNA-Coverage data from Homo Sapiens, G2cells (Tanenbaum, et al. 2015)
G1cells mRNA-Coverage data from Homo Sapiens, G1cells (Tanenbaum, et al. 2015)
Mcells mRNA-Coverage data from Homo Sapiens, Mcells (Tanenbaum, et al. 2015)

Adapter sequence CTGTAGGCACCATCAAT was removed from reads using Cutadapt. An alignment to ribosomal RNA was performed using Bowtie,and aligning reads were discarded. An alignment to the hg38 genome assembly was performed usingBowtie, and these tracks contains the uniquely mapping reads.

References

Tanenbaum ME, Stern-Ginossar N, Weissman JS, Vale RD (2015) Regulation of mRNA translation during mitosis . Science. doi: 10.7554/eLife.07957